Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Detection and comparison of HO-1 expression levels in lung tissue-derived Exos across experimental groups. The WT-COPD group exhibited significantly higher exosomal HO-1 levels than the WT-Control mice. ∗∗ p < 0.01.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Comparison, Expressing, Derivative Assay, Control

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: In vivo fluorescence imaging on Day 1 postinjection reveals distinct biodistribution of exosomes. Group I: HO-1(+/+) + PBS; Group II: HO-1(−/−) + PBS; Group III: HO-1(−/−) + WT-Con Exos; Group IV: HO-1(−/−) + WT-COPD Exos. HO-1 knockout mice were intravenously injected with PKH26-labeled Exos derived from the lungs of WT-Con or WT-COPD exacerbation mice. Representative images show that exosomes from WT-COPD exacerbation mice (Group IV) exhibited enhanced accumulation in the lungs and brain compared to those from WT-Control mice (Group III).

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: In Vivo, Fluorescence, Imaging, Knock-Out, Injection, Labeling, Derivative Assay, Control

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Biodistribution and clearance kinetics of lung-derived exosomes. Group I: HO-1(+/+) + PBS; Group II: HO-1(−/−) + PBS; Group III: HO-1(−/−) + WT-Con Exos; Group IV: HO-1(−/−) + WT-COPD Exos. (A, B) Fluorescence intensity showing higher and more sustained signals in lung tissue compared to brain tissue for both exosome types. (C) Quantification of the signal decline rate from Day 1 to Day 5, revealing significantly faster clearance from the brain in Group III. ∗ p < 0.05.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Derivative Assay, Fluorescence

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Mouse swimming paths during the Morris water maze probe trial following exogenous injections. Group I: HO-1(+/+) + PBS, Group II: HO-1(−/−) + PBS, Group III: HO-1(−/−) + WT-Con Exos, Group IV: HO-1(−/−) + WT-COPD Exos. The solid circle indicates the former platform location.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques:

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Spatial memory assessment after exogenous exosome injection. Group I: HO-1(+/+) + PBS, Group II: HO-1(−/−) + PBS, Group III: HO-1(−/−) + WT-Con Exos, Group IV: HO-1(−/−) + WT-COPD Exos. ∗ represents statistical significance at the p < 0.05. “ns” indicates p > 0.05. (a) Dwell time in the target quadrant was significantly lower in Group IV than in Group III. (b) No significant difference was observed in the number of platform crossings among all groups.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Injection

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Detection of HO-1 in blood-derived exosomes by Western blot analysis. Group I: HO-1(+/+) + PBS, Group II: HO-1(−/−) + PBS, Group III: HO-1(−/−) + WT-Con Exos, Group IV: HO-1(−/−) + WT-COPD Exos. Shown are representative protein bands from each experimental group; this is a representative blot showing qualitative expression patterns. For quantitative analysis, see .

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Derivative Assay, Western Blot, Expressing

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Detection of HO-1 in left lung tissue homogenates by Western blot analysis. Groups: I: HO-1(+/+) + PBS; II: HO-1(−/−) + PBS; III: HO-1(−/−) + WT-Con Exos; IV: HO-1(−/−) + WT-COPD Exos. Shown are representative protein bands from each experimental group.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Western Blot

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Detection of HO-1 in right lung-derived exosomes by Western blot analysis. Groups: I: HO-1(+/+) + PBS; II: HO-1(−/−) + PBS; III: HO-1(−/−) + WT-Con Exos; IV: HO-1(−/−) + WT-COPD Exos. Shown are representative protein bands from each experimental group.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Derivative Assay, Western Blot

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Detection of HO-1 in left brain tissue homogenates by Western blot analysis. Groups: I: HO-1(+/+) + PBS; II: HO-1(−/−) + PBS; III: HO-1(−/−) + WT-Con Exos; IV: HO-1(−/−) + WT-COPD Exos. Shown are representative protein bands from each experimental group.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Western Blot

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: Detection of HO-1 in right brain-derived exosomes by Western blot analysis. Groups: I: HO-1(+/+) + PBS; II: HO-1(−/−) + PBS; III: HO-1(−/−) + WT-Con Exos; IV: HO-1(−/−) + WT-COPD Exos. Shown are representative protein bands from each experimental group.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Derivative Assay, Western Blot

Journal: Canadian Respiratory Journal

Article Title: HO-1-Enriched Lung-Derived Exosomes Mediate Cognitive Impairment in a Mice Model of COPD Exacerbation

doi: 10.1155/carj/9976065

Figure Lengend Snippet: HO-1 expression is differentially regulated across tissues following exogenous exosome injection. Groups: I: HO-1(+/+) + PBS; II: HO-1(−/−) + PBS; III: HO-1(−/−) + WT-Con Exos; IV: HO-1(−/−) + WT-COPD Exos. ∗ , ∗∗ , ∗∗∗ represent statistical significance at the p < 0.05, p < 0.01, and p < 0.001 levels, respectively. “ns” indicates p > 0.05. (a) HO-1 protein levels in left lung homogenates. Group I showed higher expression than Groups III and IV. (b) HO-1 levels in right lung-derived exosomes. Expression was higher in Groups I and IV compared to Group III. (c) HO-1 levels in left brain homogenates. Expression was higher in Groups I and IV compared to Group III. (d) HO-1 levels in right brain-derived exosomes. Expression was higher in Groups I and IV compared to Group III. (e) HO-1 levels in blood-derived exosomes. Group IV showed higher expression than Groups I and III.

Article Snippet: Intriguingly, exosome (Exos) lipid bilayer extracellular vesicles (40–160 nm diameter) containing proteins, nucleic acids, and metabolites represent exceptional BBB-penetrating vectors [ ].

Techniques: Expressing, Injection, Derivative Assay